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House Dust Mites DetectionBy Estee Existence of house dirt mite often is estimated is just before in precise is identified. Request of environmental identification and operation often comes from the patients which has been diagnosed medical doctor to suffer allergy to house dirt mite. Existence of house dirt mite can be confirmation microscopically where required example of house dirt collected from mattress, sofa or carpet. House dirt antigen level besides measured from example of mattress dirt, also from example of the floor dirt and air in room. Detection at room air better be done at the time of doing sweeping activity. It is of course in this case is required a microscope with magnification that is enough and technical ability to recognize house dirt mite under microscope ( http://ohioline.osu.edu/hyg-fact/2000/2157.html). A number of complicated procedures has been publicized to dissociate house dirt mite from house dirt. A simple method to detect existence of house dirt mite is by putting down small number of house dirt above water level and investigates it under magnification of 20 times. House dirt mite live and death will be lag above water level. Furumizo ( 1973) reports a screening technique and floatation. Shamiyeh et al. ( 1973) places 0,05 dirt grams example of in 30 ml condensation NaCl saturation and adds 5 detergent drip. The suspension is rinsed through a filter with the pore diameter 45 microns, coloured with crystal violet and investigated by a stereo microscope. Hirschmann ( 1986) reports that house dirt mite interests with typewriting paper white sheet DIN A-4 placed at one particular place where anticipated by house dirt mite to stay. Paper investigated at morning and evening by holding paper under a lamp. House dirt mite will bounce a shadow http://creatures.ifas.ufl.edu/urban/house_dust_mite.htm Scanning electron micrograph from house dirt mite, Dermatophagoides farinae Hughes ( female), with magnification of 2000 times ( Photograph by GW. Wharton) ( http://creatures.ifas.ufl.edu/urban/house_dust_mite_fig2.htm For the existing has made available equipment of diagnostic test recognized by the name of Acarex sold and distributed by Fisons in United States. equipment of This diagnostic test having type dipstick is looking like equipment of glucose detector or the other which many sold in dispensary. This test can detect existence of house dirt mite faeces. A number of example of house dirt collected and mixed with available reagent with equipment of this diagnostic test. Hereinafter dipstick packed into the mixture for several time then is lifted and discoloration happened is compared to available colour tables. The result will give indication of infested level from house dirt mite. equipment of This diagnostic test must be carried through cool ( http://ohioline.osu.edu/hyg-fact/2000/2157.html). |
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