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Assessment Of Sensitization-skin Prick Test
Sensitization was assessed by the use of SPTs. The SPTs were performed and the results scored routinely (Kanerva et al 1991). The test was done on the volar aspect of the forearm. The result was read as the mean of the longest diameter of the weal and the diameter perpendicular to it. A weal diameter of 3 mm or more and equal to or greater than half of that formed by histamine hydrochloride (10 mg/ml) was defined as positive, indicating sensitization. Several enzymes were tested in studies I–V. A detailed description of the preparation of the test extract is given in publication I. In short, dry commercial enzyme preparations were extracted in 0.1 M potassium phosphate buffer, pH 7.4, and diluted to the Coca solution (0.5% sodium chloride, 0.3% sodium bicarbonate, 0.4% phenol) to achieve a protein concentration of 100 µg/ml. Part of this solution (2.5 ml) was passed through a Millex-GV filter (0.22 µm membrane, Millipore Ltd) into a sterile vial containing 2.5 ml of glycerol to yield a final protein concentration of 50 µg/ml (study I) or 100 µg/ml (studies II–V). The Coca-glycerol solution served as a negative control. Rye, wheat, barley and oat flours were tested for the bakeries and animal feed plants (studies I and IV). In the same workplaces, also storage mites were tested: Acarus siro, Lepidoglyphus destruens and Tyrophagus putrescentiae (Allergologisk Laboratorium A/S, ALK, Copenhagen, Denmark). Atopy was assessed by SPT with the following panel of common environmental allergens: cat, dog, timothy, birch, alder, mugwort, house dust mite (Dermatophagoides pteronyssinus) (ALK). A person with one or more positive SPT reactions to environmental allergens was defined as atopic.
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